Peak force tapping atomic force microscopy

Peak power tapping atomic power microscopy for advancing cell and molecular biology

The arrival of atomic power microscopy (AFM) gives an thrilling instrument to detect molecular and mobile behaviors below aqueous circumstances. AFM is ready to not solely visualize the floor topography of the specimens, but additionally can quantify the mechanical properties of the specimens by power spectroscopy assay. Nonetheless, integrating AFM topographic imaging with power spectroscopy assay has lengthy been restricted because of the low spatiotemporal decision. Lately, the looks of a brand new AFM imaging mode known as peak power tapping (PFT) has shattered this restrict. PFT permits AFM to concurrently purchase the topography and mechanical properties of organic samples with unprecedented spatiotemporal decision.

The sensible functions of PFT within the area of life sciences prior to now decade have demonstrated the superb capabilities of PFT in characterizing the high quality buildings and mechanics of dwelling organic methods of their native states, providing novel potentialities to disclose the underlying mechanisms guiding physiological/pathological actions. On this paper, the latest progress in cell and molecular biology that has been made with the utilization of PFT is summarized, and future views for additional development and biomedical functions of PFT are offered.

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BCIP (Molecular Biology Grade)

CE109 1 g
EUR 90

CHAPS (Molecular Biology Grade)

CE114 1 g
EUR 55

CHAPS (Molecular Biology Grade)

CE115 5 g
EUR 131

CHAPS (Molecular Biology Grade)

CE116 25 g
EUR 410

DAPI (Molecular Biology Grade)

CE117 5 mg
EUR 60

DAPI (Molecular Biology Grade)

CE118 25 mg
EUR 133

DAPI (Molecular Biology Grade)

CE119 100 mg
EUR 319

Dimethylsulfoxide (Molecular Biology Grade)

CE120 100 ml
EUR 55

Dimethylsulfoxide (Molecular Biology Grade)

CE121 500 ml
EUR 92

DTT (Molecular Biology Grade)

CE131 5 g
EUR 78

DTT (Molecular Biology Grade)

CE132 10 g
EUR 111

DTT (Molecular Biology Grade)

CE133 25 g
EUR 203

Glycine (Molecular Biology Grade)

CE158 1 kg
EUR 70

Glycine (Molecular Biology Grade)

CE159 5 kg
EUR 190

HEPES (Molecular Biology Grade)

CE171 100 g
EUR 82

HEPES (Molecular Biology Grade)

CE172 500 g
EUR 224

HEPES (Molecular Biology Grade)

CE173 1 kg
EUR 354

Lysozyme (Molecular Biology Grade)

CE188 1 g
EUR 59

Lysozyme (Molecular Biology Grade)

CE189 10 g
EUR 206

NAD (Molecular Biology Grade)

CE196 1 g
EUR 60

NAD (Molecular Biology Grade)

CE197 5 g
EUR 138

NBT (Molecular Biology Grade)

CE209 1 g
EUR 103

NBT (Molecular Biology Grade)

CE210 5 g
EUR 300

Tris (Molecular Biology Grade)

CE237 500 g
EUR 89

Tris (Molecular Biology Grade)

CE238 1 kg
EUR 128

Tris (Molecular Biology Grade)

CE239 5 kg
EUR 446

Tween20 (Molecular Biology Grade)

CE242 1 l
EUR 89

Water (Molecular Biology Grade)

CE243 500 ml
EUR 52

Water (Molecular Biology Grade)

CE244 1 l
EUR 56

Water, Ultrapure Molecular Biology Grade

41024-4L 4L
EUR 121
Description: Minimum order quantity: 1 unit of 4L

Ammonium sulfate (Molecular Biology Grade)

CE105 250 g
EUR 46

Ammonium sulfate (Molecular Biology Grade)

CE106 1 kg
EUR 60

Ammonium sulfate (Molecular Biology Grade)

CE107 5 kg
EUR 128

Bis-Acrylamid (Molecular Biology Grade)

CE110 50 g
EUR 79

Bis-Acrylamid (Molecular Biology Grade)

CE111 250 g
EUR 216

Formamide deionized (Molecular Biology Grade)

CE145 500 ml
EUR 73

Formamide deionized (Molecular Biology Grade)

CE146 1 l
EUR 100

Glycerol 87 % (Molecular Biology Grade)

CE154 1 l
EUR 78

Glycerol waterfree (Molecular Biology Grade)

CE155 500 ml
EUR 65

Glycerol waterfree (Molecular Biology Grade)

CE156 1 l
EUR 85

Glycerol waterfree (Molecular Biology Grade)

CE157 2.5 l
EUR 142

Guanidine - Hydrochloride (Molecular Biology Grade)

CE160 100 g
EUR 78

Guanidine - Hydrochloride (Molecular Biology Grade)

CE161 250 g
EUR 128

Guanidine - Hydrochloride (Molecular Biology Grade)

CE162 500 g
EUR 194

Guanidine - Hydrochloride (Molecular Biology Grade)

CE163 1 kg
EUR 294

Guanidine Thiocyanate (Molecular Biology Grade)

CE164 100 g
EUR 72

Guanidine Thiocyanate (Molecular Biology Grade)

CE165 500 g
EUR 160

Guanidine Thiocyanate (Molecular Biology Grade)

CE166 1 kg
EUR 256

MOPS buffer (Molecular Biology Grade)

CE194 100 g
EUR 85

MOPS buffer (Molecular Biology Grade)

CE195 250 g
EUR 141

Sodium chloride (Molecular Biology Grade)

CE205 500 g
EUR 52

Sodium chloride (Molecular Biology Grade)

CE206 1 kg
EUR 59

Sodium chloride (Molecular Biology Grade)

CE207 5 kg
EUR 103

D(+)-Sucrose (Molecular Biology Grade)

CE224 500 g
EUR 56

D(+)-Sucrose (Molecular Biology Grade)

CE225 1 kg
EUR 70

D(+)-Sucrose (Molecular Biology Grade)

CE226 5 kg
EUR 173

Tris - Hydrochloride (Molecular Biology Grade)

CE234 250 g
EUR 83

Tris - Hydrochloride (Molecular Biology Grade)

CE235 500 g
EUR 120

Tris - Hydrochloride (Molecular Biology Grade)

CE236 1 kg
EUR 186

TritonX-100 (Molecular Biology Grade)

CE240 500 ml
EUR 56

TritonX-100 (Molecular Biology Grade)

CE241 1 l
EUR 66

Tween 20, Molecular Biology Grade

T9100-010 100ml
EUR 72

Tween 20, Molecular Biology Grade

T9100-050 500ml
EUR 111

Tween 20, Molecular Biology Grade

T9100-100 1L
EUR 134

Agarose, low EEO, GlenBiol, suitable for molecular biology

GE6258-100G 100 g
EUR 181

Water, distilled, GlenBiol™, suitable for molecular biology

GK8512-1L 1 l
EUR 77

Crystalline

5-01010 10mg Ask for price

EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade)

CE135 250 g
EUR 60

EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade)

CE136 500 g
EUR 72

EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade)

CE137 1 kg
EUR 104

EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade)

CE138 5 kg
EUR 349

D(+)-Glucose waterfree (Molecular Biology Grade)

CE148 500 g
EUR 56

D(+)-Glucose waterfree (Molecular Biology Grade)

CE149 1 kg
EUR 63

D(+)-Glucose waterfree (Molecular Biology Grade)

CE150 5 kg
EUR 150

Yeast extract powder (Molecular Biology Grade)

CE169 500 g
EUR 111

Hyaluronidase Grade I (Molecular Biology Grade)

CE174 1 g
EUR 194

Hyaluronidase Grade I (Molecular Biology Grade)

CE175 5 g
EUR 767

Magnesium acetate - Tetrahydrate (Molecular Biology Grade)

CE190 500 g
EUR 82

NADH - Disodium salt (Molecular Biology Grade)

CE198 1 g
EUR 76

NADH - Disodium salt (Molecular Biology Grade)

CE199 5 g
EUR 204

NADP - sodium salt (Molecular Biology Grade)

CE200 250 mg
EUR 77

NADP - sodium salt (Molecular Biology Grade)

CE201 1 g
EUR 159

NADPH - Tetrasodium salt (Molecular Biology Grade)

CE202 25 mg
EUR 59

NADPH - Tetrasodium salt (Molecular Biology Grade)

CE203 100 mg
EUR 105

NADPH - Tetrasodium salt (Molecular Biology Grade)

CE204 500 mg
EUR 312

SSC Buffer (20X) (Molecular Biology Grade)

CE229 1 l
EUR 72

XTT sodium salt (Molecular Biology Grade)

CE250 100 mg
EUR 174

XTT sodium salt (Molecular Biology Grade)

CE251 500 mg
EUR 510

Albumin fraction V (pH7,0) (Molecular Biology Grade)

CE100 50 g
EUR 107

Albumin fraction V (pH7,0) (Molecular Biology Grade)

CE101 100 g
EUR 161

Albumin fraction V (pH7,0) (Molecular Biology Grade)

CE102 250 g
EUR 323

Albumin fraction V (pH7,0) (Molecular Biology Grade)

CE103 500 g
EUR 547

Albumin fraction V (pH7,0) (Molecular Biology Grade)

CE104 1 kg
EUR 969

Agarose LE, Ultra-Pure Molecular Biology Grade, 100 g

41028-100G 100G
EUR 222
Description: Minimum order quantity: 1 unit of 100G

Agarose LE, Ultra-Pure Molecular Biology Grade, 25 g

41028-25G 25G
EUR 109
Description: Minimum order quantity: 1 unit of 25G

Agarose LE, Ultra-Pure Molecular Biology Grade, 500 g

41028-500G 500G
EUR 719
Description: Minimum order quantity: 1 unit of 500G

EDTA solution pH 8.0 (0.5 M) (Molecular Biology Grade)

CE141 500 ml
EUR 73

LB-Agar - Powder according to Lennox (Molecular Biology Grade)

CE178 500 g
EUR 91

LB-Agar - Powder according to Lennox (Molecular Biology Grade)

CE179 2.5 kg
EUR 251

LB-Agar - Powder according to Miller (Molecular Biology Grade)

CE180 500 g
EUR 87

LB-Agar - Powder according to Miller (Molecular Biology Grade)

CE181 2.5 kg
EUR 246

LB-Medium - Powder according to Lennox (Molecular Biology Grade)

CE182 500 g
EUR 90

LB-Medium - Powder according to Lennox (Molecular Biology Grade)

CE183 2.5 kg
EUR 251

LB-Medium - Powder according to Miller (Molecular Biology Grade)

CE184 2.5 kg
EUR 246

AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 100G PER UNIT

AGR-LE-100 1/pk
EUR 168
Description: Bioscience Mol Bio; Agarose

Phenol

20-abx184753
  • EUR 244.00
  • EUR 217.00
  • 1 kg
  • 500 g

100 ML, MOLECULAR BIOLOGY GRADE WATER; TESTED TO USP STERILE PURIFIED WATER SPECIFICATIONS

46-000-CI 100 mL/pk
EUR 57
Description: Media Catalog; Sterile Wi-Fi Qual, Cell

1 L, MOLECULAR BIOLOGY GRADE WATER; TESTED TO USP STERILE PURIFIED WATER SPECIFICATIONS

46-000-CM 1L/pk
EUR 98
Description: Media Catalog; Sterile Wi-Fi Qual, Cell

500 ML, MOLECULAR BIOLOGY GRADE WATER; TESTED TO USP STERILE PURIFIED WATER SPECIFICATIONS

46-000-CV 500 mL/pk
EUR 106
Description: Media Catalog; Sterile Wi-Fi Qual, Cell

AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 500G/UNIT, 10 UNITS PER CASE

AGR-LE-5001 1/pk
EUR 3058
Description: Bioscience Mol Bio; Agarose

AGAROSE MS, PCR SCREENING, MOLECULAR BIOLOGY GRADE, 100G/UNIT, 10 UNITS PER CASE

AGR-MS-1001 1/pk
EUR 2038
Description: Bioscience Mol Bio; Agarose

AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 100G PER UNIT, 10 UNITS PER CASE

AGR-LE-1001 1/pk
EUR 772
Description: Bioscience Mol Bio; Agarose

AGAROSE LM, LOW MELTING, MOLECULAR BIOLOGY GRADE, 100G/UNIT, 10 UNITS PER CASE (AL)

AGR-LM-1001 1/pk
EUR 3737
Description: Bioscience Mol Bio; Agarose

AGAROSE LM, LOW MELTING, MOLECULAR BIOLOGY GRADE, 50G/UNIT, 10 UNITS PER CASE (AL)

AGR-LM-501 1/pk
EUR 2075
Description: Bioscience Mol Bio; Agarose

Crystalline [H-Trp-Gly-OH; MW: 261.2]

SP-55342-1 1 mg
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Nonyl Phenol

AT037 1mg
EUR 1368

Nonyl Phenol

AG037 1 mg
EUR 523

Water Phenol

abx082109-250ml 250 ml
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Phenol, 99%

GK6990-100G 100 g
EUR 44

Phenol, 99%

GK6990-1KG 1 kg
EUR 78

Phenol, 99%

GK6990-500G 500 g
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Phenol Red

GT9844-100G 100 g
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GT9844-250G 250 g
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Phenol Red

GT9844-25G 25 g
EUR 62

Phenol Red

GT9844-5G 5 g
EUR 46

Phenol red

PB0420 25g
EUR 70.88

ELISA kit for Rat PST (Phenol Sulfotransferase)

ELK6740 1 plate of 96 wells
EUR 432
Description: A sandwich ELISA kit for detection of Phenol Sulfotransferase from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

ELISA kit for Human PST (Phenol Sulfotransferase)

ELK3246 1 plate of 96 wells
EUR 432
Description: A sandwich ELISA kit for detection of Phenol Sulfotransferase from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

ELISA kit for Mouse PST (Phenol Sulfotransferase)

ELK3642 1 plate of 96 wells
EUR 432
Description: A sandwich ELISA kit for detection of Phenol Sulfotransferase from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

3-(Aminomethyl)phenol

20-abx185751
  • EUR 425.00
  • EUR 2277.00
  • EUR 857.00
  • 1 g
  • 25 g
  • 5 g

Phenol, 4-propoxy-

20-abx184303
  • EUR 523.00
  • EUR 286.00
  • 125g
  • 25 g

2-(Aminomethyl)phenol

20-abx185699
  • EUR 314.00
  • EUR 1442.00
  • EUR 592.00
  • 1 g
  • 25 g
  • 5 g

4-(Benzyloxy)phenol

GP9986-100G 100 g
EUR 118

4-(Benzyloxy)phenol

GP9986-250G 250 g
EUR 198

4-(Benzyloxy)phenol

GP9986-25G 25 g
EUR 59

Phenol Red Solution

CA013-010 100ml
EUR 69

Phenol, saturated (pH7.9)

PC6910 100ml
EUR 76.1

Phenol, saturated (pH4.5)

PC6920 100ml
EUR 76.1

30UL CLEAR PIPET TIPS FOR MOLECULAR DEVICE LIBERTY.

LT-384-R 3840/pk
EUR 2193
Description: Robotic Tips; Robotic tips - Axygen

Molecular Weight Marker

abx098957-15ul 15 ul
EUR 105

Agarose, Molecular Grade

BIO-41025 500g Ask for price

Agarose, Molecular Grade

BIO-41026 100g Ask for price

Recombinant Phenol Sulfotransferase (PST)

4-RPB268Hu01
  • EUR 481.70
  • EUR 232.00
  • EUR 1531.36
  • EUR 577.12
  • EUR 1054.24
  • EUR 385.00
  • EUR 3678.40
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Phenol Sulfotransferase expressed in: E.coli

Recombinant Phenol Sulfotransferase (PST)

4-RPB268Mu01
  • EUR 490.66
  • EUR 234.00
  • EUR 1564.96
  • EUR 588.32
  • EUR 1076.64
  • EUR 391.00
  • EUR 3762.40
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Phenol Sulfotransferase expressed in: E.coli

Recombinant Phenol Sulfotransferase (PST)

4-RPB268Ra01
  • EUR 510.37
  • EUR 239.00
  • EUR 1638.88
  • EUR 612.96
  • EUR 1125.92
  • EUR 404.00
  • EUR 3947.20
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Phenol Sulfotransferase expressed in: E.coli

PHENOL RED BROTH BASE

P16-104-10kg 10 kg
EUR 899

PHENOL RED BROTH BASE

P16-104-2kg 2kg
EUR 234

PHENOL RED BROTH BASE

P16-104-500g 500 g
EUR 100

PHENOL RED DEXTROSE BROTH

P16-105-10kg 10 kg
EUR 743

PHENOL RED DEXTROSE BROTH

P16-105-2Kg 2 Kg
EUR 200

PHENOL RED DEXTROSE BROTH

P16-105-500g 500 g
EUR 90

PHENOL RED LACTOSE BROTH

P16-121-10kg 10 kg
EUR 1254

PHENOL RED LACTOSE BROTH

P16-121-2Kg 2 Kg
EUR 312

PHENOL RED LACTOSE BROTH

P16-121-500g 500 g
EUR 121

PHENOL RED MANNITOL BROTH

P16-122-10kg 10 kg
EUR 1563

PHENOL RED MANNITOL BROTH

P16-122-2kg 2kg
EUR 379

PHENOL RED MANNITOL BROTH

P16-122-500g 500 g
EUR 139

PHENOL RED SUCROSE BROTH

P16-123-10kg 10 kg
EUR 835

PHENOL RED SUCROSE BROTH

P16-123-2Kg 2 Kg
EUR 221

PHENOL RED SUCROSE BROTH

P16-123-500g 500 g
EUR 96

Secretome Profiling and Computational Biology of Human Leiomyoma Samples Unravel Molecular Signatures with Potential for Diagnostic and Therapeutic Interventions

Lately, vital developments have been made in the best way the complicated proteome samples are in contrast however the final word objective of routine biomarker discovery has but to be achieved. Primarily based on reverse genetic technique, our examine concerned the recognizing of genes exhibiting expressional variability in uterine leiomyoma females. Serum samples have been taken from uterine leiomyomas topics (n=6) and wholesome management topics (n=6) for proteomic research. Moreover, leiomyoma tissue samples (n=25) and regular myometrium samples (n=25) have been taken for validation research. On this examine, we profiled the proteomes of uterine leiomyoma affected person’s serum and wholesome management, together with relative quantification utilizing Nano LC-MS/MS evaluation. A complete of 146 proteins have been reported to be considerably differentially expressed (P worth lower than 0.05) in case and management pattern. Statistical evaluation recognized plenty of molecular signatures distinguishing wholesome from diseased serum.

Amongst these, 5 proteins lumican, ficolin, MASP2, EMSY, and kallistatin have been additional chosen based on their operate for validation. Kallistatin was downregulated whereas ficolin, MASP2, lumican, and EMSY have been discovered to be upregulated within the diseased pattern. The expression modulations within the recognized genes have been additional validated in twenty-five instances. Interactions among the many differentially expressed proteins have been recognized adopted with community evaluation. Community evaluation emphasised vital pathways which are extremely deregulated in myoma, and practical significance of those pathways within the pathology of the illness was mentioned. Comparative expression evaluation reveals distinct molecular signatures and their possible function in prognosis of the illness.

Excessive grade gliomas in younger youngsters: The South Thames Neuro-Oncology unit expertise and up to date advances in molecular biology and focused therapies

Excessive grade gliomas (HGG) have a dismal prognosis with survival charges of 15-35%. Roughly 10-12% of pediatric HGG happen in younger youngsters and their molecular biology and medical outcomes differ from these arising at older ages. We report on 4 youngsters aged <5 years newly recognized with non-brainstem HGG between 2011 and 2018 who have been handled with surgical procedure and BBSFOP chemotherapy. Two died of tumor development. The opposite two are nonetheless alive with out radiotherapy at 3.eight and three.9 years from prognosis: considered one of whom stays disease-free off therapy; and the opposite one, whose tumor harbored a KCTD16:NTRK2 fusion, went on to obtain larotrectinib.

Moreover we assessment the final administration, outcomes and newest updates in molecular biology and focused therapies for younger youngsters with HGG. Toddler gliomas may be stratified in molecular subgroups with clinically actionable oncogenic drivers. Chemotherapy-based methods can keep away from or delay the necessity for radiotherapy in younger youngsters with HGG. Harnessing the potential of NTRK, ALK, ROS1 and MET inhibitors gives the chance to optimize the therapeutic armamentarium to enhance present outcomes for these youngsters.

Poisonous stays: Infrastructural failure in a Ugandan molecular biology lab

This text complicates romances of infrastructural improvisation by describing infrastructural failures that expose researchers to hazardous chemical substances in a Ugandan molecular biology lab. To fulfill mission deadlines, to make careers and to take part in transnational collaborative initiatives, Ugandan biologists have to face in for decaying or absent infrastructures with their our bodies.

Ugandan biologists conceal such sacrifices from their worldwide scientific companions and direct the blame elsewhere. An unclear culpability outcomes exactly from the methods by which energy works and is distributed throughout transnational scientific infrastructures.

Growth of a Certification Examination to Assess Undergraduate College students’ Proficiency in Biochemistry and Molecular Biology Core Ideas

With assist from the American Society for Biochemistry and Molecular Biology (ASBMB), a group of biochemistry and molecular biology (BMB) scientist-educators has developed and administered an evaluation instrument designed to judge scholar competence throughout 4 core idea and ability areas elementary to BMB. The 4 areas embody power and metabolism; data storage and switch; macromolecular construction, operate, and meeting; and expertise together with analytical and quantitative reasoning.

First supplied in 2014, the examination has now been administered to almost 4000 college students in ASBMB-accredited applications at greater than 70 schools and universities. Right here, we describe the event and continued maturation of the examination program, together with the natural function of college volunteers as drivers and stewards of all aspects: content material and format choice, query improvement, and scoring.

Instructing molecular strategies at residence: Molecular biology labs that may be carried out wherever and allow hands-on studying of restriction digestion/ligation and DNA amplification

COVID 19 has modified about each side of life together with how we train in larger training. Laboratory experiments important for studying hands-on strategies are restricted because of social distancing necessities and elevated numbers of distance-learning college students. The answer to lack of hands-on actions has been to compensate with digital laboratory modules.

Though digital labs are partaking and provide a simulated hands-on method to instructing important molecular strategies, these simulations don’t exchange hands-on expertise. I designed two molecular biology laboratory workouts in response to the present instructing limitations that may be accomplished ‘at-home’ and allow low value hands-on instruction of important molecular strategies in any distance-learning surroundings together with through the COVID 19 pandemic.

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