Molecular Biology Strategies in Streptomyces rimosus, a Producer of Oxytetracycline
Streptomyces rimosus is used for manufacturing of the broad-spectrum antibiotic oxytetracycline (OTC). S. rimosus belongs to Actinomyces species, a big group of microorganisms that produce various set of pure metabolites of excessive significance in lots of points of our life. On this chapter, we describe particular molecular biology strategies and a classical homologous recombination method for focused in-frame deletion of a goal gene or complete operon in S. rimosus genome. The introduced protocols will information you thru the design of experiment and building of homology arms and their cloning into acceptable vectors, that are appropriate for gene-engineering work with S. rimosus. Moreover, two totally different protocols for S. rimosus transformation are described together with detailed process for focused gene substitute by way of double crossover recombination occasion.
Gene deletion is confirmed by colony PCR, and colonies are additional characterised by cultivation and metabolite evaluation. As the ultimate step, we current in trans complementation of the deleted gene, to verify performance of the engineering method achieved by gene disruption. A lot of methodological steps and protocols are optimized for S. rimosus strains together with the usage of the chosen reporter genes. Protocols described on this chapter may be utilized for learning operate of any particular person gene product in various OTC-producing Streptomyces rimosus strains.
Urea, suitable for molecular biology |
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GE1210-1KG | Glentham Life Sciences | 1 kg | EUR 106.8 |
Urea, suitable for molecular biology |
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GE1210-500 | Glentham Life Sciences | 500 | EUR 33.1 |
Urea, suitable for molecular biology |
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GE1210-500G | Glentham Life Sciences | 500 g | EUR 76.8 |
2-Mercaptoethanol ?For Molecular Biology |
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MB041-100ML | EWC Diagnostics | 1 unit | EUR 9.02 |
Description: 2-Mercaptoethanol ?For Molecular Biology |
2-Mercaptoethanol ?For Molecular Biology |
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MB041-500ML | EWC Diagnostics | 1 unit | EUR 26.47 |
Description: 2-Mercaptoethanol ?For Molecular Biology |
Sodium chloride, suitable for molecular biology |
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GE0307-1 | Glentham Life Sciences | 1 | EUR 45.2 |
Molecular Biology Grade Water for RT-PCR |
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ML065-1.5ML | EWC Diagnostics | 1 unit | EUR 7.82 |
Description: Molecular Biology Grade Water for RT-PCR |
Pyridine, GlenBiol™, suitable for molecular biology with molecular sieve |
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GS8780-2500 | Glentham Life Sciences | 2500 | EUR 249.8 |
DTT (Molecular Biology Grade) |
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CE131 | GeneOn | 5 g | EUR 93.6 |
DTT (Molecular Biology Grade) |
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CE132 | GeneOn | 10 g | EUR 133.2 |
DTT (Molecular Biology Grade) |
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CE133 | GeneOn | 25 g | EUR 243.6 |
NAD (Molecular Biology Grade) |
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CE196 | GeneOn | 1 g | EUR 72 |
NAD (Molecular Biology Grade) |
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CE197 | GeneOn | 5 g | EUR 165.6 |
NBT (Molecular Biology Grade) |
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CE209 | GeneOn | 1 g | EUR 123.6 |
NBT (Molecular Biology Grade) |
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CE210 | GeneOn | 5 g | EUR 360 |
DMSO, Molecular Biology Grade |
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40470006-1 | Glycomatrix | 100 mL | EUR 88.18 |
DMSO, Molecular Biology Grade |
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40470006-2 | Glycomatrix | 250 mL | EUR 150.19 |
DMSO, Molecular Biology Grade |
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40470006-3 | Glycomatrix | 500 mL | EUR 279.26 |
EGTA, Molecular Biology Grade |
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40500028-2 | Glycomatrix | 50 g | EUR 106.43 |
EGTA, Molecular Biology Grade |
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40500028-3 | Glycomatrix | 100 g | EUR 177.58 |
EGTA, Molecular Biology Grade |
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40500028-4 | Glycomatrix | 500 g | EUR 603.19 |
EGTA, Molecular Biology Grade |
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40500028-5 | Glycomatrix | 1 kg | EUR 912.98 |
EGTA, Molecular Biology Grade |
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40500028-6 | Glycomatrix | 2 kg | EUR 1687.94 |
BCIP (Molecular Biology Grade) |
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CE108 | GeneOn | 250 mg | EUR 75.6 |
BCIP (Molecular Biology Grade) |
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CE109 | GeneOn | 1 g | EUR 108 |
DAPI (Molecular Biology Grade) |
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CE117 | GeneOn | 5 mg | EUR 72 |
DAPI (Molecular Biology Grade) |
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CE118 | GeneOn | 25 mg | EUR 159.6 |
DAPI (Molecular Biology Grade) |
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CE119 | GeneOn | 100 mg | EUR 382.8 |
Tris (Molecular Biology Grade) |
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CE237 | GeneOn | 500 g | EUR 106.8 |
Tris (Molecular Biology Grade) |
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CE238 | GeneOn | 1 kg | EUR 153.6 |
Tris (Molecular Biology Grade) |
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CE239 | GeneOn | 5 kg | EUR 535.2 |
Pyridine, GlenBiol™, suitable for molecular biology |
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GS6659-2500 | Glentham Life Sciences | 2500 | EUR 240.3 |
Pyridine, GlenBiol™, suitable for molecular biology |
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GS6659-500 | Glentham Life Sciences | 500 | EUR 95.8 |
Formamide, GlenBiol™, suitable for molecular biology |
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GS9663-100 | Glentham Life Sciences | 100 | EUR 48.9 |
CHAPS (Molecular Biology Grade) |
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CE114 | GeneOn | 1 g | EUR 66 |
CHAPS (Molecular Biology Grade) |
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CE115 | GeneOn | 5 g | EUR 157.2 |
CHAPS (Molecular Biology Grade) |
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CE116 | GeneOn | 25 g | EUR 492 |
HEPES (Molecular Biology Grade) |
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CE171 | GeneOn | 100 g | EUR 98.4 |
HEPES (Molecular Biology Grade) |
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CE172 | GeneOn | 500 g | EUR 268.8 |
HEPES (Molecular Biology Grade) |
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CE173 | GeneOn | 1 kg | EUR 424.8 |
Water (Molecular Biology Grade) |
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CE243 | GeneOn | 500 ml | EUR 62.4 |
Water (Molecular Biology Grade) |
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CE244 | GeneOn | 1 l | EUR 67.2 |
Molecular Biology Grade Water |
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ML024-100ML | EWC Diagnostics | 1 unit | EUR 3.54 |
Description: Molecular Biology Grade Water |
Molecular Biology Grade Water |
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ML024-10X100ML | EWC Diagnostics | 1 unit | EUR 29.52 |
Description: Molecular Biology Grade Water |
Molecular Biology Grade Water |
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ML024-10X500ML | EWC Diagnostics | 1 unit | EUR 87.91 |
Description: Molecular Biology Grade Water |
Molecular Biology Grade Water |
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ML024-500ML | EWC Diagnostics | 1 unit | EUR 12.56 |
Description: Molecular Biology Grade Water |
Molecular Biology Grade Water |
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ML064-100ML | EWC Diagnostics | 1 unit | EUR 3.67 |
Description: Molecular Biology Grade Water |
Molecular Biology Grade Water |
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ML064-10X100ML | EWC Diagnostics | 1 unit | EUR 25.38 |
Description: Molecular Biology Grade Water |
Molecular Biology Grade Water |
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ML064-500ML | EWC Diagnostics | 1 unit | EUR 10.81 |
Description: Molecular Biology Grade Water |
Agarose, Molecular Biology Grade |
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40100164-1 | Bio-WORLD | 25 g | Ask for price |
Agarose, Molecular Biology Grade |
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40100164-2 | Bio-WORLD | 50 g | Ask for price |
Agarose, Molecular Biology Grade |
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40100164-3 | Bio-WORLD | 100 g | Ask for price |
Agarose, Molecular Biology Grade |
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40100164-4 | Bio-WORLD | 500 g | Ask for price |
Agarose, Molecular Biology Grade |
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40100164-5 | Bio-WORLD | 1 kg | Ask for price |
Glycine (Molecular Biology Grade) |
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CE158 | GeneOn | 1 kg | EUR 84 |
Glycine (Molecular Biology Grade) |
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CE159 | GeneOn | 5 kg | EUR 228 |
Tween20 (Molecular Biology Grade) |
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CE242 | GeneOn | 1 l | EUR 106.8 |
Agarose (Molecular Biology Grade) |
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abx299715-100g | Abbexa | 100 µg | Ask for price |
Agarose (Molecular Biology Grade) |
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abx299715-20g | Abbexa | 20 µg | EUR 525 |
Agarose (Molecular Biology Grade) |
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abx299715-50g | Abbexa | 50 µg | Ask for price |
Lysozyme (Molecular Biology Grade) |
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CE188 | GeneOn | 1 g | EUR 70.8 |
Lysozyme (Molecular Biology Grade) |
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CE189 | GeneOn | 10 g | EUR 247.2 |
Lysozyme (Molecular Biology Grade) |
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CE189L | GeneOn | 50 g | EUR 310 |
Lysozyme (Molecular Biology Grade) |
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CE189XL | GeneOn | 250 g | EUR 1050 |
OORA00229-1L - Molecular Biology Grade UltraPure Water |
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OORA00229-1L | Aviva Systems Biology | 1L | EUR 149 |
OORA00230-1L - Molecular Biology Grade UltraPure Water |
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OORA00230-1L | Aviva Systems Biology | 1L | EUR 279 |
Dimethylformamide, GlenBiol™, suitable for molecular biology with molecular sieve |
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GS3406-2500 | Glentham Life Sciences | 2500 | EUR 116.2 |
"10X PBS, Molecular Biology Grade" |
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ML023-100ML | EWC Diagnostics | 1 unit | EUR 17.65 |
Description: "10X PBS, Molecular Biology Grade" |
10X PBS, Molecular Biology Grade |
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ML023-500ML | EWC Diagnostics | 1 unit | EUR 38.97 |
Description: 10X PBS, Molecular Biology Grade |
"10X TBS, Molecular Biology Grade" |
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ML029-100ML | EWC Diagnostics | 1 unit | EUR 19.04 |
Description: "10X TBS, Molecular Biology Grade" |
"10X TBS, Molecular Biology Grade" |
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ML029-500ML | EWC Diagnostics | 1 unit | EUR 40.48 |
Description: "10X TBS, Molecular Biology Grade" |
10X TBS, Molecular Biology Grade |
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ML029-6X500ML | EWC Diagnostics | 1 unit | EUR 138.56 |
Description: 10X TBS, Molecular Biology Grade |
Agarose, low EEO, GlenBiol, suitable for molecular biology |
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GE6258-100G | Glentham Life Sciences | 100 g | EUR 217.2 |
OORA00218-100IU - DNA Ligase T4 Molecular Biology Grade |
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OORA00218-100IU | Aviva Systems Biology | 100Units | EUR 129 |
Tween 20, Molecular Biology Grade |
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T9100-010 | GenDepot | 100ml | EUR 86.4 |
Tween 20, Molecular Biology Grade |
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T9100-050 | GenDepot | 500ml | EUR 133.2 |
Tween 20, Molecular Biology Grade |
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T9100-100 | GenDepot | 1L | EUR 160.8 |
Dimethylformamide, GlenBiol™, suitable for molecular biology |
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GS6580-2500 | Glentham Life Sciences | 2500 | EUR 107.3 |
100mL Molecular Biology Grade - PK6 |
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46-000-CI | Scientific Laboratory Supplies | PK6 | EUR 83.7 |
500mL Molecular Biology Grade - PK6 |
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46-000-CV | Scientific Laboratory Supplies | PK6 | EUR 155.25 |
Boric Acid, Molecular Biology Grade |
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40200060-1 | Glycomatrix | 500 g | EUR 46.16 |
Boric Acid, Molecular Biology Grade |
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40200060-2 | Glycomatrix | 1 kg | EUR 76.66 |
Boric Acid, Molecular Biology Grade |
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40200060-3 | Glycomatrix | 2.5 kg | EUR 145.5 |
20xSSC, Molecular Biology Grade, pH7.0 |
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TBS5033-1L | Tribioscience | 1L | EUR 67 |
Water, distilled, GlenBiol™, suitable for molecular biology |
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GK8512-1 | Glentham Life Sciences | 1 | EUR 60.1 |
Water, distilled, GlenBiol™, suitable for molecular biology |
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GK8512-1L | Glentham Life Sciences | 1 l | EUR 92.4 |
Acetonitrile 50, GlenBiol™, suitable for molecular biology |
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GS0247-1 | Glentham Life Sciences | 1 | EUR 40.7 |
Acetonitrile 50, GlenBiol™, suitable for molecular biology |
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GS0247-2500 | Glentham Life Sciences | 2500 | EUR 61.3 |
Acetonitrile 10, GlenBiol™, suitable for molecular biology |
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GS0969-1 | Glentham Life Sciences | 1 | EUR 47 |
Acetonitrile 10, GlenBiol™, suitable for molecular biology |
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GS0969-2500 | Glentham Life Sciences | 2500 | EUR 73.9 |
Acetonitrile 30, GlenBiol™, suitable for molecular biology |
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GS8649-1 | Glentham Life Sciences | 1 | EUR 43.1 |
Acetonitrile 30, GlenBiol™, suitable for molecular biology |
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GS8649-2500 | Glentham Life Sciences | 2500 | EUR 66.5 |
OORA00218-100UNITS - DNA Ligase T4 Molecular Biology Grade |
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OORA00218-100UNITS | Aviva Systems Biology | 100Units | EUR 149 |
Formamide deionized (Molecular Biology Grade) |
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CE145 | GeneOn | 500 ml | EUR 87.6 |
Formamide deionized (Molecular Biology Grade) |
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CE146 | GeneOn | 1 l | EUR 120 |
RNase/DNase free water, for molecular biology (10 x 1.5ml) |
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Z-RNase/DNase-free-water | Novacyt Group | n/a | EUR 62 |
Glycerol 87 % (Molecular Biology Grade) |
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CE154 | GeneOn | 1 l | EUR 93.6 |
MOPS buffer (Molecular Biology Grade) |
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CE194 | GeneOn | 100 g | EUR 102 |
MOPS buffer (Molecular Biology Grade) |
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CE195 | GeneOn | 250 g | EUR 169.2 |
Agarose, Molecular Biology Grade, 100g |
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PC0701-100g | Vivantis | each | Ask for price |
Agarose, Molecular Biology Grade, 1kg |
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PC0701-1kg | Vivantis | each | Ask for price |
Agarose, Molecular Biology Grade, 500g |
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PC0701-500g | Vivantis | each | Ask for price |
Phenol, (Carbolic acid) Double distilled for Molecular Biology |
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PD0252 | Bio Basic | 500g | EUR 192.59 |
Ammonia solution, GlenBiol™, suitable for molecular biology |
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GS8853-100 | Glentham Life Sciences | 100 | EUR 91.2 |
Dimethylsulfoxide (Molecular Biology Grade) |
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CE120 | GeneOn | 100 ml | EUR 66 |
Dimethylsulfoxide (Molecular Biology Grade) |
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CE121 | GeneOn | 500 ml | EUR 110.4 |
Agarose, low EEO, GlenBiol™, suitable for molecular biology |
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GE6258-100 | Glentham Life Sciences | 100 | EUR 150.4 |
Agarose, low EEO, GlenBiol™, suitable for molecular biology |
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GE6258-25 | Glentham Life Sciences | 25 | EUR 60.1 |
TritonX-100 (Molecular Biology Grade) |
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CE240 | GeneOn | 500 ml | EUR 67.2 |
TritonX-100 (Molecular Biology Grade) |
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CE241 | GeneOn | 1 l | EUR 79.2 |
OORA00219-v1 - DNA Ligase T4 Molecular Biology Grade (500units) |
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OORA00219-v1 | Aviva Systems Biology | 500units | EUR 375 |
Cesium Chloride, Molecular Biology Grade |
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40300060-1 | Glycomatrix | 50 g | EUR 45.77 |
Water, Ultrapure Molecular Biology Grade |
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41024-4L | Biotium | 4L | EUR 79 |
Description: N/A |
Water, Ultrapure Molecular Biology Grade |
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41024-4L-1 | Biotium | EA | EUR 79 |
Bis-Acrylamid (Molecular Biology Grade) |
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CE110 | GeneOn | 50 g | EUR 94.8 |
Bis-Acrylamid (Molecular Biology Grade) |
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CE111 | GeneOn | 250 g | EUR 259.2 |
Sodium chloride (Molecular Biology Grade) |
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CE205 | GeneOn | 500 g | EUR 62.4 |
Sodium chloride (Molecular Biology Grade) |
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CE206 | GeneOn | 1 kg | EUR 70.8 |
Sodium chloride (Molecular Biology Grade) |
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CE207 | GeneOn | 5 kg | EUR 123.6 |
1L Molecular Biology Grade Water - PK6 |
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46-000-CM | Scientific Laboratory Supplies | PK6 | EUR 221.4 |
Past descriptive accuracy: The central dogma of molecular biology in scientific follow
There isn’t a denying the Central Dogma’s influence on the organic sciences. Because the Dogma’s formulation by Francis Crick in 1958, nonetheless, many have debated the Dogma’s empirical adequacy. My goal is to maneuver past these discussions, and as a substitute take into account the Central Dogma’s significance to modern organic follow.
To do that, I take into account 4 distinct approaches for figuring out the non-descriptive methodological significance of a scientific precept. I argue that these approaches fail to vindicate the Central Dogma, and that, below many of those approaches, the Dogma quantities to a triviality.
Molecular Biology of Bladder Most cancers: Potential Implications for Remedy
Lately accomplished research offered high-resolution descriptions of the molecular organic traits of urothelial bladder cancers. Complete transcriptome messenger RNA expression profiling revealed that they are often grouped into basal and luminal molecular subtypes resembling those described in breast cancers.
Retrospective DNA sequencing efforts revealed roles for disruption of DNA injury response pathways in response to standard chemotherapy and immune checkpoint blockade, and accomplished and ongoing research point out that the molecular organic properties of infiltrating host cells dictate additionally affect therapeutic outcomes. This text opinions these findings and establish gaps in information that characterize alternatives for future analysis.
A guided-inquiry investigation of genetic variants utilizing Oxford nanopore sequencing for an undergraduate molecular biology laboratory course
Subsequent Era Sequencing (NGS) has grow to be an necessary instrument within the organic sciences and has a rising variety of purposes throughout medical fields. Presently, few undergraduate packages present coaching within the design and implementation of NGS purposes. Right here, we describe an inquiry-based laboratory train for a college-level molecular biology laboratory course that makes use of real-time MinION deep sequencing and bioinformatics to research attribute genetic variants present in most cancers cell-lines. The general objective for college students was to establish non-small cell lung most cancers (NSCLC) cell-lines primarily based on their distinctive genomic profiles.
The models described on this laboratory spotlight core rules in multiplex PCR primer design, real-time deep sequencing, and bioinformatics evaluation for genetic variants. We discovered that the MinION machine is an acceptable, possible instrument that gives a complete, hands-on NGS expertise for undergraduates. Pupil evaluations demonstrated elevated confidence in utilizing molecular methods and enhanced understanding of NGS ideas. General, this train gives a pedagogical instrument for incorporating NGS approaches within the educating laboratory as manner of enhancing college students’ comprehension of genomic sequence evaluation. Additional, this NGS lab module can simply be added to a wide range of lab-based programs to assist undergraduate college students be taught present DNA sequencing strategies with restricted effort and price.
Prioritizing Molecular Biomarkers in Bronchial asthma and Respiratory Allergy Utilizing Programs Biology
Extremely prevalent respiratory ailments reminiscent of bronchial asthma and allergy stay a urgent well being problem. Presently, there may be an unmet want for exact diagnostic instruments able to predicting the nice heterogeneity of those diseases. In a earlier research of 94 bronchial asthma/respiratory allergy biomarker candidates, we outlined a bunch of potential biomarkers to differentiate medical phenotypes (i.e. nonallergic bronchial asthma, allergic bronchial asthma, respiratory allergy with out bronchial asthma) and illness severity. Right here, we analyze our experimental outcomes utilizing advanced algorithmic approaches that set up holistic illness fashions (programs biology), combining these insights with info obtainable in specialised databases developed worldwide. With this method, we goal to prioritize probably the most related biomarkers in keeping with their specificity and mechanistic implication with molecular motifs of the ailments. The Therapeutic Efficiency Mapping System (Anaxomics’ TPMS know-how) was used to generate one mathematical mannequin per illness: allergic bronchial asthma (AA), non-allergic bronchial asthma (NA), and respiratory allergy (RA), defining particular molecular motifs for every.
The connection of our molecular biomarker candidates and every illness was analyzed by synthetic neural networks (ANNs) scores. These analyses prioritized molecular biomarkers particular to the ailments and to explicit molecular motifs. As a primary step, molecular characterization of the pathophysiological processes of AA outlined 16 molecular motifs: 2 particular for AA, 2 shared with RA, and 12 shared with NA. Mechanistic evaluation confirmed 17 proteins that had been strongly associated to AA. Eleven proteins had been related to RA and 16 proteins with NA. Specificity evaluation confirmed that 12 proteins had been particular to AA, 7 had been particular to RA, and a couple of to NA. Lastly, a triggering evaluation revealed a related function for AKT1, STAT1, and MAPK13 in all three situations and for TLR4 in asthmatic ailments (AA and NA). In conclusion, this research has enabled us to prioritize biomarkers relying on the performance related to every illness and with particular molecular motifs, which may enhance the definition and usefulness of recent molecular biomarkers.
Molecular Biology of Ovarian Most cancers: From Mechanisms of Intraperitoneal Metastasis to Therapeutic Alternatives
Ovarian most cancers (OC) is likely one of the most frequent malignancies of the feminine genital tract, and continues to be the main explanation for loss of life from gynecological tumors […].
Tris - Hydrochloride (Molecular Biology Grade) |
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CE235 | GeneOn | 500 g | EUR 144 |
Tris - Hydrochloride (Molecular Biology Grade) |
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CE236 | GeneOn | 1 kg | EUR 223.2 |
Guanidine thiocyanate?For Molecular Biol |
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MB015-1KG | EWC Diagnostics | 1 unit | EUR 118.81 |
Description: Guanidine thiocyanate?For Molecular Biol |
Guanidine thiocyanate?For Molecular Biol |
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MB015-500G | EWC Diagnostics | 1 unit | EUR 79.2 |
Description: Guanidine thiocyanate?For Molecular Biol |
Guanidine Hydrochloride |
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09860-25 | NACALAI TESQUE | 500G | EUR 224 |
Guanidine Hydrochloride |
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17318-24 | NACALAI TESQUE | 10KG | EUR 742 |
Guanidine Hydrochloride |
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17318-82 | NACALAI TESQUE | 25G | EUR 14 |
Guanidine Hydrochloride |
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17318-95 | NACALAI TESQUE | 500G | EUR 68.6 |
Guanidine Hydrochloride |
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17319-14 | NACALAI TESQUE | 100G | EUR 32.2 |
Guanidine Hydrochloride |
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17319-85 | NACALAI TESQUE | 500G | EUR 84 |
Guanidine Hydrochloride |
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17353-25 | NACALAI TESQUE | 500G | EUR 147 |
Guanidine Hydrochloride |
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18-206 | Genesee Scientific | 500g/Unit | EUR 191.53 |
Description: Molecular/Proteomic Grade |
Guanidine Hydrochloride |
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40700060-1 | Glycomatrix | 100 g | EUR 36.33 |
Guanidine Hydrochloride |
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40700060-2 | Glycomatrix | 500 g | EUR 89.21 |
Guanidine Hydrochloride |
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40700060-3 | Glycomatrix | 1 kg | EUR 153.37 |
Guanidine Hydrochloride |
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G821245 | Toronto Research Chemicals | 10g | EUR 63 |
Description: 50-01-1 |
Guanidine hydrochloride |
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GB0242 | Bio Basic | 100g | EUR 80.88 |
Guanidine Hydrochloride |
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A2719-100G | Biomatik Corporation | 100G | EUR 30.8 |
Description: Biotechnology |
Guanidine Hydrochloride |
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A2719-500G | Biomatik Corporation | 500G | EUR 97.9 |
Description: Biotechnology |
Guanidine (hydrochloride) |
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HY-B0178A | MedChemExpress | 50g | EUR 159.6 |
Guanidine hydrochloride |
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T0415-10mg | TargetMol Chemicals | 10mg | Ask for price |
Description: Guanidine hydrochloride |
Guanidine hydrochloride |
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T0415-1g | TargetMol Chemicals | 1g | Ask for price |
Description: Guanidine hydrochloride |
Guanidine hydrochloride |
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T0415-1mg | TargetMol Chemicals | 1mg | Ask for price |
Description: Guanidine hydrochloride |
Guanidine hydrochloride |
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T0415-50mg | TargetMol Chemicals | 50mg | Ask for price |
Description: Guanidine hydrochloride |
Guanidine hydrochloride |
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T0415-5mg | TargetMol Chemicals | 5mg | Ask for price |
Description: Guanidine hydrochloride |
Guanidine hydrochloride, 99% |
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GE1914-1 | Glentham Life Sciences | 1 | EUR 79.1 |
Guanidine hydrochloride, 99% |
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GE1914-100 | Glentham Life Sciences | 100 | EUR 15.9 |
Guanidine hydrochloride, 99% |
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GE1914-100G | Glentham Life Sciences | 100 g | EUR 55.2 |
Guanidine hydrochloride, 99% |
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GE1914-1KG | Glentham Life Sciences | 1 kg | EUR 132 |
Guanidine hydrochloride, 99% |
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GE1914-25 | Glentham Life Sciences | 25 | EUR 13 |
Guanidine hydrochloride, 99% |
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GE1914-250 | Glentham Life Sciences | 250 | EUR 23.8 |
Guanidine hydrochloride, 99% |
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GE1914-250G | Glentham Life Sciences | 250 g | EUR 64.8 |
Guanidine hydrochloride, 99% |
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GE1914-25G | Glentham Life Sciences | 25 g | EUR 50.4 |
Guanidine hydrochloride, 99% |
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GE1914-500 | Glentham Life Sciences | 500 | EUR 43.5 |
Guanidine hydrochloride, 99% |
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GE1914-500G | Glentham Life Sciences | 500 g | EUR 88.8 |
Guanidine hydrochloride powder |
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G05320 | Pfaltz & Bauer | 100G | EUR 126.5 |
Guanidine-15N3 Hydrochloride |
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G821249 | Toronto Research Chemicals | 25mg | EUR 1200 |
Description: 121616-39-5 |
Guanidine hydrochloride solution, 8M |
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GE1001-100 | Glentham Life Sciences | 100 | EUR 120.5 |
Guanidine hydrochloride solution, 8M |
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GE1001-500 | Glentham Life Sciences | 500 | EUR 421.8 |
Guanidine hydrochloride solution, 6M |
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GE4739-1 | Glentham Life Sciences | 1 | EUR 711.9 |
Guanidine hydrochloride solution, 6M |
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GE4739-100 | Glentham Life Sciences | 100 | EUR 118.7 |
Guanidine hydrochloride solution, 6M |
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GE4739-500 | Glentham Life Sciences | 500 | EUR 408.1 |
Guanidine hydrochloride, Hi-LR™ |
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GRM1504-100G | EWC Diagnostics | 1 unit | EUR 46.61 |
Description: Guanidine hydrochloride, Hi-LR™ |
Guanidine hydrochloride, Hi-LR™ |
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GRM1504-25G | EWC Diagnostics | 1 unit | EUR 13.32 |
Description: Guanidine hydrochloride, Hi-LR™ |
Guanidine hydrochloride (6 M) solution |
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B1013-1L | Biovision | each | EUR 301.2 |
Guanidine hydrochloride (6 M) solution |
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B1013-4L | Biovision | each | EUR 705.6 |
1-(2,4-difluorophenyl)guanidine hydrochloride |
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T50030-10mg | TargetMol Chemicals | 10mg | Ask for price |
Description: 1-(2,4-difluorophenyl)guanidine hydrochloride |
1-(2,4-difluorophenyl)guanidine hydrochloride |
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T50030-1g | TargetMol Chemicals | 1g | Ask for price |
Description: 1-(2,4-difluorophenyl)guanidine hydrochloride |
1-(2,4-difluorophenyl)guanidine hydrochloride |
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T50030-1mg | TargetMol Chemicals | 1mg | Ask for price |
Description: 1-(2,4-difluorophenyl)guanidine hydrochloride |
1-(2,4-difluorophenyl)guanidine hydrochloride |
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T50030-50mg | TargetMol Chemicals | 50mg | Ask for price |
Description: 1-(2,4-difluorophenyl)guanidine hydrochloride |
1-(2,4-difluorophenyl)guanidine hydrochloride |
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T50030-5mg | TargetMol Chemicals | 5mg | Ask for price |
Description: 1-(2,4-difluorophenyl)guanidine hydrochloride |
N-(3,4-Dimethoxyphenyl)guanidine Hydrochloride |
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D470625 | Toronto Research Chemicals | 10g | EUR 800 |
Description: 855890-38-9 |
N-Cyano-N'-(6-guanidinohexyl) Guanidine Hydrochloride |
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C655610 | Toronto Research Chemicals | 250mg | EUR 400 |
N-(4-Methyl-2-thiazolyl)-guanidine hydrochloride |
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M331435 | Toronto Research Chemicals | 100mg | EUR 58 |
Description: 100599-91-5 |
N-(6-Aminohexyl)-N'-(6-guanidinohexyl) Guanidine Hydrochloride |
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A544590 | Toronto Research Chemicals | 50mg | EUR 6000 |
Casein, for Molecular Biology |
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MB279-100G | EWC Diagnostics | 1 unit | EUR 110.77 |
Description: Casein, for Molecular Biology |
Casein, for Molecular Biology |
|||
MB279-500G | EWC Diagnostics | 1 unit | EUR 387.65 |
Description: Casein, for Molecular Biology |
1-(4-Chloromethyl-2-thiazoyl)guanidine Hydrochloride Salt |
|||
C369545 | Toronto Research Chemicals | 1g | EUR 146 |
Urea, suitable for molecular biology |
|||
GE1210-1 | Glentham Life Sciences | 1 | EUR 58 |
Urea, suitable for molecular biology |
|||
GE1210-1KG | Glentham Life Sciences | 1 kg | EUR 106.8 |
Urea, suitable for molecular biology |
|||
GE1210-500 | Glentham Life Sciences | 500 | EUR 33.1 |
Urea, suitable for molecular biology |
|||
GE1210-500G | Glentham Life Sciences | 500 g | EUR 76.8 |
2-Mercaptoethanol ?For Molecular Biology |
|||
MB041-100ML | EWC Diagnostics | 1 unit | EUR 9.02 |
Description: 2-Mercaptoethanol ?For Molecular Biology |
2-Mercaptoethanol ?For Molecular Biology |
|||
MB041-500ML | EWC Diagnostics | 1 unit | EUR 26.47 |
Description: 2-Mercaptoethanol ?For Molecular Biology |
Sodium chloride, suitable for molecular biology |
|||
GE0307-1 | Glentham Life Sciences | 1 | EUR 45.2 |
Sucrose, GlenBiol, suitable for molecular biology |
|||
GC3201-1KG | Glentham Life Sciences | 1 kg | EUR 90 |
Molecular Biology Grade Water for RT-PCR |
|||
ML065-1.5ML | EWC Diagnostics | 1 unit | EUR 7.82 |
Description: Molecular Biology Grade Water for RT-PCR |
Pyridine, GlenBiol™, suitable for molecular biology with molecular sieve |
|||
GS8780-2500 | Glentham Life Sciences | 2500 | EUR 249.8 |
DTT (Molecular Biology Grade) |
|||
CE131 | GeneOn | 5 g | EUR 93.6 |
DTT (Molecular Biology Grade) |
|||
CE132 | GeneOn | 10 g | EUR 133.2 |
DTT (Molecular Biology Grade) |
|||
CE133 | GeneOn | 25 g | EUR 243.6 |
NAD (Molecular Biology Grade) |
|||
CE196 | GeneOn | 1 g | EUR 72 |
NAD (Molecular Biology Grade) |
|||
CE197 | GeneOn | 5 g | EUR 165.6 |
NBT (Molecular Biology Grade) |
|||
CE209 | GeneOn | 1 g | EUR 123.6 |
NBT (Molecular Biology Grade) |
|||
CE210 | GeneOn | 5 g | EUR 360 |
Sucrose, GlenBiol™, suitable for molecular biology |
|||
GC3201-1 | Glentham Life Sciences | 1 | EUR 45.1 |
EGTA, Molecular Biology Grade |
|||
40500028-2 | Glycomatrix | 50 g | EUR 106.43 |
EGTA, Molecular Biology Grade |
|||
40500028-3 | Glycomatrix | 100 g | EUR 177.58 |
EGTA, Molecular Biology Grade |
|||
40500028-4 | Glycomatrix | 500 g | EUR 603.19 |
EGTA, Molecular Biology Grade |
|||
40500028-5 | Glycomatrix | 1 kg | EUR 912.98 |
EGTA, Molecular Biology Grade |
|||
40500028-6 | Glycomatrix | 2 kg | EUR 1687.94 |
DMSO, Molecular Biology Grade |
|||
40470006-1 | Glycomatrix | 100 mL | EUR 88.18 |
DMSO, Molecular Biology Grade |
|||
40470006-2 | Glycomatrix | 250 mL | EUR 150.19 |
DMSO, Molecular Biology Grade |
|||
40470006-3 | Glycomatrix | 500 mL | EUR 279.26 |
BCIP (Molecular Biology Grade) |
|||
CE108 | GeneOn | 250 mg | EUR 75.6 |
BCIP (Molecular Biology Grade) |
|||
CE109 | GeneOn | 1 g | EUR 108 |
DAPI (Molecular Biology Grade) |
|||
CE117 | GeneOn | 5 mg | EUR 72 |
DAPI (Molecular Biology Grade) |
|||
CE118 | GeneOn | 25 mg | EUR 159.6 |
DAPI (Molecular Biology Grade) |
|||
CE119 | GeneOn | 100 mg | EUR 382.8 |
Tris (Molecular Biology Grade) |
|||
CE237 | GeneOn | 500 g | EUR 106.8 |
Tris (Molecular Biology Grade) |
|||
CE238 | GeneOn | 1 kg | EUR 153.6 |
Tris (Molecular Biology Grade) |
|||
CE239 | GeneOn | 5 kg | EUR 535.2 |
Pyridine, GlenBiol™, suitable for molecular biology |
|||
GS6659-2500 | Glentham Life Sciences | 2500 | EUR 240.3 |
Pyridine, GlenBiol™, suitable for molecular biology |
|||
GS6659-500 | Glentham Life Sciences | 500 | EUR 95.8 |
Formamide, GlenBiol™, suitable for molecular biology |
|||
GS9663-100 | Glentham Life Sciences | 100 | EUR 48.9 |
CHAPS (Molecular Biology Grade) |
|||
CE114 | GeneOn | 1 g | EUR 66 |
CHAPS (Molecular Biology Grade) |
|||
CE115 | GeneOn | 5 g | EUR 157.2 |
CHAPS (Molecular Biology Grade) |
|||
CE116 | GeneOn | 25 g | EUR 492 |
HEPES (Molecular Biology Grade) |
|||
CE171 | GeneOn | 100 g | EUR 98.4 |
HEPES (Molecular Biology Grade) |
|||
CE172 | GeneOn | 500 g | EUR 268.8 |
HEPES (Molecular Biology Grade) |
|||
CE173 | GeneOn | 1 kg | EUR 424.8 |
Water (Molecular Biology Grade) |
|||
CE243 | GeneOn | 500 ml | EUR 62.4 |
Water (Molecular Biology Grade) |
|||
CE244 | GeneOn | 1 l | EUR 67.2 |
Molecular Biology Grade Water |
|||
ML024-100ML | EWC Diagnostics | 1 unit | EUR 3.54 |
Description: Molecular Biology Grade Water |
Molecular Biology Grade Water |
|||
ML024-10X100ML | EWC Diagnostics | 1 unit | EUR 29.52 |
Description: Molecular Biology Grade Water |
Molecular Biology Grade Water |
|||
ML024-10X500ML | EWC Diagnostics | 1 unit | EUR 87.91 |
Description: Molecular Biology Grade Water |
Molecular Biology Grade Water |
|||
ML024-500ML | EWC Diagnostics | 1 unit | EUR 12.56 |
Description: Molecular Biology Grade Water |
Molecular Biology Grade Water |
|||
ML064-100ML | EWC Diagnostics | 1 unit | EUR 3.67 |
Description: Molecular Biology Grade Water |
Molecular Biology Grade Water |
|||
ML064-10X100ML | EWC Diagnostics | 1 unit | EUR 25.38 |
Description: Molecular Biology Grade Water |
Molecular Biology Grade Water |
|||
ML064-500ML | EWC Diagnostics | 1 unit | EUR 10.81 |
Description: Molecular Biology Grade Water |
Agarose, Molecular Biology Grade |
|||
40100164-1 | Bio-WORLD | 25 g | Ask for price |
Agarose, Molecular Biology Grade |
|||
40100164-2 | Bio-WORLD | 50 g | Ask for price |
Agarose, Molecular Biology Grade |
|||
40100164-3 | Bio-WORLD | 100 g | Ask for price |
Agarose, Molecular Biology Grade |
|||
40100164-4 | Bio-WORLD | 500 g | Ask for price |
Agarose, Molecular Biology Grade |
|||
40100164-5 | Bio-WORLD | 1 kg | Ask for price |
Glycine (Molecular Biology Grade) |
|||
CE158 | GeneOn | 1 kg | EUR 84 |
Glycine (Molecular Biology Grade) |
|||
CE159 | GeneOn | 5 kg | EUR 228 |
Tween20 (Molecular Biology Grade) |
|||
CE242 | GeneOn | 1 l | EUR 106.8 |
Agarose (Molecular Biology Grade) |
|||
abx299715-100g | Abbexa | 100 µg | Ask for price |
Agarose (Molecular Biology Grade) |
|||
abx299715-20g | Abbexa | 20 µg | EUR 525 |
Agarose (Molecular Biology Grade) |
|||
abx299715-50g | Abbexa | 50 µg | Ask for price |
Lysozyme (Molecular Biology Grade) |
|||
CE188 | GeneOn | 1 g | EUR 70.8 |
Lysozyme (Molecular Biology Grade) |
|||
CE189 | GeneOn | 10 g | EUR 247.2 |
Lysozyme (Molecular Biology Grade) |
|||
CE189L | GeneOn | 50 g | EUR 310 |
Lysozyme (Molecular Biology Grade) |
|||
CE189XL | GeneOn | 250 g | EUR 1050 |
OORA00229-1L - Molecular Biology Grade UltraPure Water |
|||
OORA00229-1L | Aviva Systems Biology | 1L | EUR 149 |
OORA00230-1L - Molecular Biology Grade UltraPure Water |
|||
OORA00230-1L | Aviva Systems Biology | 1L | EUR 279 |
Dimethylformamide, GlenBiol™, suitable for molecular biology with molecular sieve |
|||
GS3406-2500 | Glentham Life Sciences | 2500 | EUR 116.2 |
"10X PBS, Molecular Biology Grade" |
|||
ML023-100ML | EWC Diagnostics | 1 unit | EUR 17.65 |
Description: "10X PBS, Molecular Biology Grade" |
10X PBS, Molecular Biology Grade |
|||
ML023-500ML | EWC Diagnostics | 1 unit | EUR 38.97 |
Description: 10X PBS, Molecular Biology Grade |
"10X TBS, Molecular Biology Grade" |
|||
ML029-100ML | EWC Diagnostics | 1 unit | EUR 19.04 |
Description: "10X TBS, Molecular Biology Grade" |
"10X TBS, Molecular Biology Grade" |
|||
ML029-500ML | EWC Diagnostics | 1 unit | EUR 40.48 |
Description: "10X TBS, Molecular Biology Grade" |
10X TBS, Molecular Biology Grade |
|||
ML029-6X500ML | EWC Diagnostics | 1 unit | EUR 138.56 |
Description: 10X TBS, Molecular Biology Grade |
Agarose, low EEO, GlenBiol, suitable for molecular biology |
|||
GE6258-100G | Glentham Life Sciences | 100 g | EUR 217.2 |
OORA00218-100IU - DNA Ligase T4 Molecular Biology Grade |
|||
OORA00218-100IU | Aviva Systems Biology | 100Units | EUR 129 |
Tween 20, Molecular Biology Grade |
|||
T9100-010 | GenDepot | 100ml | EUR 86.4 |
Tween 20, Molecular Biology Grade |
|||
T9100-050 | GenDepot | 500ml | EUR 133.2 |
Tween 20, Molecular Biology Grade |
|||
T9100-100 | GenDepot | 1L | EUR 160.8 |
Dimethylformamide, GlenBiol™, suitable for molecular biology |
|||
GS6580-2500 | Glentham Life Sciences | 2500 | EUR 107.3 |
100mL Molecular Biology Grade - PK6 |
|||
46-000-CI | Scientific Laboratory Supplies | PK6 | EUR 83.7 |
500mL Molecular Biology Grade - PK6 |
|||
46-000-CV | Scientific Laboratory Supplies | PK6 | EUR 155.25 |
Boric Acid, Molecular Biology Grade |
|||
40200060-1 | Glycomatrix | 500 g | EUR 46.16 |
Boric Acid, Molecular Biology Grade |
|||
40200060-2 | Glycomatrix | 1 kg | EUR 76.66 |
Boric Acid, Molecular Biology Grade |
|||
40200060-3 | Glycomatrix | 2.5 kg | EUR 145.5 |
D(+)-Sucrose (Molecular Biology Grade) |
|||
CE224 | GeneOn | 500 g | EUR 67.2 |
D(+)-Sucrose (Molecular Biology Grade) |
|||
CE225 | GeneOn | 1 kg | EUR 84 |
D(+)-Sucrose (Molecular Biology Grade) |
|||
CE226 | GeneOn | 5 kg | EUR 207.6 |
20xSSC, Molecular Biology Grade, pH7.0 |
|||
TBS5033-1L | Tribioscience | 1L | EUR 67 |
Acetonitrile 50, GlenBiol™, suitable for molecular biology |
|||
GS0247-1 | Glentham Life Sciences | 1 | EUR 40.7 |
Acetonitrile 50, GlenBiol™, suitable for molecular biology |
|||
GS0247-2500 | Glentham Life Sciences | 2500 | EUR 61.3 |
Acetonitrile 10, GlenBiol™, suitable for molecular biology |
|||
GS0969-1 | Glentham Life Sciences | 1 | EUR 47 |
Acetonitrile 10, GlenBiol™, suitable for molecular biology |
|||
GS0969-2500 | Glentham Life Sciences | 2500 | EUR 73.9 |
Acetonitrile 30, GlenBiol™, suitable for molecular biology |
|||
GS8649-1 | Glentham Life Sciences | 1 | EUR 43.1 |
Acetonitrile 30, GlenBiol™, suitable for molecular biology |
|||
GS8649-2500 | Glentham Life Sciences | 2500 | EUR 66.5 |
Water, distilled, GlenBiol™, suitable for molecular biology |
|||
GK8512-1 | Glentham Life Sciences | 1 | EUR 60.1 |
Water, distilled, GlenBiol™, suitable for molecular biology |
|||
GK8512-1L | Glentham Life Sciences | 1 l | EUR 92.4 |
OORA00218-100UNITS - DNA Ligase T4 Molecular Biology Grade |
|||
OORA00218-100UNITS | Aviva Systems Biology | 100Units | EUR 149 |
Formamide deionized (Molecular Biology Grade) |
|||
CE145 | GeneOn | 500 ml | EUR 87.6 |
Formamide deionized (Molecular Biology Grade) |
|||
CE146 | GeneOn | 1 l | EUR 120 |
RNase/DNase free water, for molecular biology (10 x 1.5ml) |
|||
Z-RNase/DNase-free-water | Novacyt Group | n/a | EUR 62 |
Glycerol 87 % (Molecular Biology Grade) |
|||
CE154 | GeneOn | 1 l | EUR 93.6 |
MOPS buffer (Molecular Biology Grade) |
|||
CE194 | GeneOn | 100 g | EUR 102 |
MOPS buffer (Molecular Biology Grade) |
|||
CE195 | GeneOn | 250 g | EUR 169.2 |
Agarose, Molecular Biology Grade, 100g |
|||
PC0701-100g | Vivantis | each | Ask for price |
Agarose, Molecular Biology Grade, 1kg |
|||
PC0701-1kg | Vivantis | each | Ask for price |
Agarose, Molecular Biology Grade, 500g |
|||
PC0701-500g | Vivantis | each | Ask for price |
Ammonia solution, GlenBiol™, suitable for molecular biology |
|||
GS8853-100 | Glentham Life Sciences | 100 | EUR 91.2 |
Phenol, (Carbolic acid) Double distilled for Molecular Biology |
|||
PD0252 | Bio Basic | 500g | EUR 192.59 |
Dimethylsulfoxide (Molecular Biology Grade) |
|||
CE120 | GeneOn | 100 ml | EUR 66 |
Dimethylsulfoxide (Molecular Biology Grade) |
|||
CE121 | GeneOn | 500 ml | EUR 110.4 |
Agarose, low EEO, GlenBiol™, suitable for molecular biology |
|||
GE6258-100 | Glentham Life Sciences | 100 | EUR 150.4 |
Agarose, low EEO, GlenBiol™, suitable for molecular biology |
|||
GE6258-25 | Glentham Life Sciences | 25 | EUR 60.1 |
TritonX-100 (Molecular Biology Grade) |
|||
CE240 | GeneOn | 500 ml | EUR 67.2 |
TritonX-100 (Molecular Biology Grade) |
|||
CE241 | GeneOn | 1 l | EUR 79.2 |
OORA00219-v1 - DNA Ligase T4 Molecular Biology Grade (500units) |
|||
OORA00219-v1 | Aviva Systems Biology | 500units | EUR 375 |
Water, Ultrapure Molecular Biology Grade |
|||
41024-4L | Biotium | 4L | EUR 79 |
Description: N/A |
Water, Ultrapure Molecular Biology Grade |
|||
41024-4L-1 | Biotium | EA | EUR 79 |
Bis-Acrylamid (Molecular Biology Grade) |
|||
CE110 | GeneOn | 50 g | EUR 94.8 |
Bis-Acrylamid (Molecular Biology Grade) |
|||
CE111 | GeneOn | 250 g | EUR 259.2 |
Sodium chloride (Molecular Biology Grade) |
|||
CE205 | GeneOn | 500 g | EUR 62.4 |
Sodium chloride (Molecular Biology Grade) |
|||
CE206 | GeneOn | 1 kg | EUR 70.8 |
Sodium chloride (Molecular Biology Grade) |
|||
CE207 | GeneOn | 5 kg | EUR 123.6 |
Cesium Chloride, Molecular Biology Grade |
|||
40300060-1 | Glycomatrix | 50 g | EUR 45.77 |
1L Molecular Biology Grade Water - PK6 |
|||
46-000-CM | Scientific Laboratory Supplies | PK6 | EUR 221.4 |
Lithium Chloride, Molecular Biology Grade |
|||
41200036-1 | Glycomatrix | 100 g | EUR 41.7 |
Lithium Chloride, Molecular Biology Grade |
|||
41200036-2 | Glycomatrix | 500 g | EUR 108.38 |
Ammonium sulfate (Molecular Biology Grade) |
|||
CE105 | GeneOn | 250 g | EUR 55.2 |
Ammonium sulfate (Molecular Biology Grade) |
|||
CE106 | GeneOn | 1 kg | EUR 72 |
Ammonium sulfate (Molecular Biology Grade) |
|||
CE107 | GeneOn | 5 kg | EUR 153.6 |
Urea Crystalline (Molecular Biology Grade) |
|||
CE167 | GeneOn | 1 kg | EUR 72 |